The Europe-focused STOXX 600 index fell 1.1%, with automobile stocks leading losses with a 2.2% drop.
Finnish tyre maker Nokian Tyres was the worst performer in the sector, down 4.4% amid investor disappointment with the firm's new margin targets.
Economy-sensitive financial services, oil & gas, and banking stocks fell around 1.3%, as investors feared any changes to the ECB's large stimulus program, given a recent spike in inflation.
"It's only a month ago that stocks were at all-time highs, and with an ECB meeting tomorrow, people will be unwilling to buy because it can be a bit of a risky trade," said David Madden, market analyst at Equiti Capital in London.
"It's more of what happened in the U.S. last night and just a bit of position squaring ahead of ECB."
The central bank is expected to announce a reduction in bond buying on Thursday, with analysts polled by Reuters forecasting purchases under the Pandemic Emergency Purchase Programme (PEPP) falling possibly as low as 60 billion euros ($70.91 billion) a month from the current 80 billion euros.
Austria's central bank chief Robert Holzmann, considered a hawkish member of the ECB, also said the central bank could tighten policy sooner than many expect as inflationary pressures could prove to be persistent.
After hitting a record high in mid-August, the STOXX 600 has hovered below those levels as worries over central bank policies and signs of slowing global growth kept investors on edge.
Spanish turbine maker Siemens Gamesa fell 8.6%, and was the worst performer on the STOXX 600 after JPMorgan downgraded the stock to a "neutral" rating. Danish peer Vestas also fell 4.5%.
Swedish investment company EQT fell 3.4% after a share-placing deal, while Stellantis dropped 2.9% as Dongfeng Motor Hong Kong said it had sold shares in the carmaker for about 600 million euros ($710 million).
French drugmaker Sanofi slipped 2.5% after it agreed to buy U.S. biopharmaceutical company Kadmon Holdings Inc in a $1.9 billion deal.
British industrial technology company Smiths Group rose 2.6% after it agreed to sell its medical unit to U.S.-based ICU Medical Inc for $2.4 billion.
($1 = 0.8461 euros)
(Reporting by Sruthi Shankar in Bengaluru; Editing by Saumyadeb Chakrabarty, Uttaresh.V and Mark Heinrich)
By Sruthi Shankar and Ambar Warrick